Peptide Companion
Peptide Companion
blend
Recovery & Repair
Vial: 20 mg

How is BPC-157 + TB-500 (20 mg Blend) dosed and mixed?

BPC-157 + TB-500 (20 mg Blend) is a peptide blend research reference in the Recovery & Repair category for a 20 mg vial. The reference mixing guide adds 3 mL of bacteriostatic water, giving a concentration of 6.67 mg/mL. The published research schedule lists BPC daily, TB-500 twice weekly over 6–8 weeks; it is reference information, not a recommendation.

By Vincent Ceniceros · Published · Last updated

BPC-157 (Body Protection Compound-157) is a 15-amino-acid peptide fragment derived from human gastric juice, studied for its cytoprotective and tissue-repair properties across gastrointestinal, musculoskeletal, and neurological systems. TB-500 (Thymosin β4) is a 43-amino-acid peptide involved in cell migration, angiogenesis, and wound repair. This blend combines both peptides for synergistic tissue-repair research applications.

Dose
As per individual components
Frequency
BPC daily, TB-500 twice weekly
Timing
BPC morning/evening, TB-500 any time
Duration
6–8 weeks

Synergistic repair effects. Pre-mixed for convenience. Addresses both vascular and cellular repair.

Dosing Schedule
WeekDaily Dose (total blend)Per-Peptide DoseUnits (mL)
Weeks 1–2500 mcg (0.5 mg)~250 mcg each7.5 units (0.075 mL)
Weeks 3–4666 mcg (0.67 mg)~333 mcg each10 units (0.10 mL)
Weeks 5–81,000 mcg (1.0 mg)~500 mcg each15 units (0.15 mL)
Mixing & Quick Reference
Bacteriostatic water
3 mL
Concentration
6.67 mg/mL
  • •Reconstitute: Add 3.0 mL bacteriostatic water → ~ 6.67 mg/mL total concentration (~3.33 mg/mL of each peptide).
  • •Typical daily range: 250–500 mcg BPC-157 \+ 250–500 mcg TB-500 (500–1,000 mcg total blend) once daily.
  • •Easy measuring: At 6.67 mg/mL total, 1 unit = 0.01 mL ≈ 66.7 mcg total (~33.3 mcg of each peptide) on a U-100 insulin syringe.
  • •Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); use within 14 days.
Supplies Needed
  • •Peptide Vials (BPC-157 + TB-500 20 mg Blend):
  • •Insulin Syringes (U-100):
  • •Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution.
  • •Alcohol Swabs: One for the vial stopper + one for the injection site each day.
Benefits
  • •Tissue Repair: Both peptides demonstrate wound-repair, tendon-repair, and anti-inflammatory properties in animal models.
  • •Gastrointestinal Support: BPC-157 shows gastroprotective and ulcer-repair effects in preclinical studies.
  • •Safety Profile: Preclinical data indicate a wide safety margin for BPC-157 (no lethal dose up to ~20 mg/kg); TB-500 human tolerability studies report mild adverse events at doses up to 1,260 mg.
  • •Possible Side Effects: Occasional mild injection-site reactions (redness, itching); rare reports of transient dizziness or nausea; long-term human safety data remain limited.
How It Works

BPC-157 is a synthetic pentadecapeptide (15 amino acids) originally isolated from human gastric juice. Preclinical research indicates it exerts cytoprotective effects across multiple organ systems, promotes angiogenesis, modulates nitric oxide pathways, and interacts with growth-factor systems involved in tissue repair. TB-500 (Thymosin β4) is a naturally occurring peptide that regulates actin polymerization, promotes cell migration, reduces inflammation, and supports wound repair and tissue regeneration. The combination is hypothesized to provide complementary mechanisms for accelerated musculoskeletal and soft-tissue recovery.

References and original studies

  • Biomedicines (2022)

    Stable gastric pentadecapeptide BPC 157: pleiotropic beneficial activity and neurotransmitter interactions

    View original study

  • Current Pharmaceutical Design (2018)

    BPC 157 and standard angiogenic growth factors: gastrointestinal tract healing and angiogenesis

    View original study

  • Annals of the New York Academy of Sciences (2007)

    Thymosin β4: roles in development, repair, and regeneration

    View original study

  • Expert Opinion on Biological Therapy (2018)

    Thymosin β4: a multi-functional regenerative peptide; basic mechanisms and clinical applications

    View original study

  • Bachem Technical Note

    Peptide stability and handling guidelines

    View source

  • International Journal of Clinical Pharmacy (2013)

    Evaluation of maximum beyond-use-date stability of peptide solutions

    View original study

  • CDC

    Vaccine administration: subcutaneous route (angle, site rotation guidance)

    View source

  • WADA Prohibited List (2024)

    Growth factors and peptides prohibited in sport

    View source

  • Arthroscopy (2025)

    Emerging use of BPC-157 in orthopaedic sports medicine: a systematic review

    View original study

  • PubMed (2010)

    Randomized, placebo-controlled, single and multiple dose study of intravenous thymosin beta4 in healthy volunteers

    View original study

  • CDC (Subcut Injection PDF)

    Technique diagram and site guidance for subcutaneous injections

    View source

  • NCBI Bookshelf

    Administration of parenteral medications: best practices

    View original study

  • Subcutaneous Drug Injection Review (PMC)

    Pharmacologic considerations of the subcutaneous route

    View original study

About the author

Vincent Ceniceros

Vincent Ceniceros created Peptide Companion and publishes its Dosage Library. Each guide summarizes a reference protocol and links to the studies and sources it draws on.

Published · Last updated

Related guides

Work out the mixing math for any vial with the peptide reconstitution calculator, or browse the full Dosage Library.

Educational content adapted from peptidedosages.com. Not medical advice.