Peptide Companion
Peptide Companion
blend
Recovery & Repair
Vial: 45 mg

How is Tri-Heal (45 mg Blend) dosed and mixed?

Tri-Heal (45 mg Blend) is a peptide blend research reference in the Recovery & Repair category for a 45 mg vial. The reference mixing guide adds 3 mL of bacteriostatic water, giving a concentration of 15 mg/mL. The published research schedule lists 0.25–0.5 mg per injection, once or twice daily for 4–8 weeks; it is reference information, not a recommendation.

By Vincent Ceniceros · Published · Last updated

Tri-Heal is a research peptide blend combining TB-500 (25 mg), BPC-157 (10 mg), and KPV (10 mg) for advanced tissue repair and inflammation modulation. TB-500 is a synthetic fragment of thymosin β4, a 43-amino-acid peptide involved in cell migration, angiogenesis, and wound repair. BPC-157 is a synthetic 15-amino-acid body protection compound with pro-angiogenic and gastroprotective properties. KPV (Lys-Pro-Val) is a tripeptide derived from α-MSH noted for potent anti-inflammatory effects. This educational protocol presents a once-daily subcutaneous approach using a practical dilution for clear insulin-syringe measurements.

Dose
0.25–0.5 mg per injection
Frequency
Once or twice daily
Timing
Morning and/or evening
Duration
4–8 weeks

Systemic repair effects. Can be injected near injury site or subcutaneously. Promotes angiogenesis.

Dosing Schedule
WeekDaily Dose (mcg)Daily Dose (mg)Units (per injection)Volume (mL)
Weeks 1–2500 mcg0.5 mg3.3 units\*0.033 mL
Weeks 3–41000 mcg1.0 mg6.7 units\*0.067 mL
Weeks 5–61500 mcg1.5 mg10 units0.10 mL
Weeks 7–82000 mcg2.0 mg13.3 units0.133 mL
Mixing & Quick Reference
Bacteriostatic water
3 mL
Concentration
15 mg/mL
  • •Reconstitute: Add 3.0 mL bacteriostatic water → 15 mg/mL concentration (15,000 mcg/mL).
  • •Typical daily range: 500–2000 mcg once daily (gradual titration over 8 weeks).
  • •Easy measuring: At 15 mg/mL, 1 unit = 0.01 mL = 150 mcg on a U-100 insulin syringe.
  • •Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.
Supplies Needed
  • •Peptide Vials (Tri-Heal, 45 mg each): - 8 weeks (56 doses, ~70 mg total): 2 vials - 12 weeks (84 doses, ~147 mg total): 4 vials - 16 weeks (112 doses, ~252 mg total): 6 vials
  • •Insulin Syringes (U-100):
  • •Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution. - 8 weeks (2 vials): 6 mL → 1 × 10 mL bottle - 12 weeks (4 vials): 12 mL → 2 × 10 mL bottles - 16 weeks (6 vials): 18 mL → 2 × 10 mL bottles
  • •Alcohol Swabs: One for the vial stopper + one for the injection site each day. - Per week: 14 swabs (2/day) - 8 weeks: 112 swabs → recommend 2 × 100-count boxes - 12 weeks: 168 swabs → recommend 2 × 100-count boxes - 16 weeks: 224 swabs → recommend 3 × 100-count boxes
Benefits
  • •TB-500: Promotes cell proliferation, angiogenesis, and accelerated tissue repair; inhibits apoptosis and inflammation in repair tissues.
  • •BPC-157: Accelerates musculoskeletal repair including tendon and muscle repair; stimulates endothelial regeneration and fibroblast activity.
  • •KPV: Reduces inflammatory cytokine release; demonstrates efficacy in models of epithelial inflammation.
  • •General tolerability: Individual peptides are generally well tolerated in preclinical studies; occasional mild injection-site reactions (redness, transient discomfort) may occur with subcutaneous administration.
How It Works

Tri-Heal combines three peptides with complementary mechanisms targeting multiple aspects of tissue injury repair:

TB-500 (Thymosin β4): Acts as a natural repair factor, inhibiting inflammation and apoptosis while promoting angiogenesis, cell migration, and matrix remodeling. This 43-amino-acid peptide has been shown to accelerate dermal repair in both preclinical models and clinical applications.

BPC-157 (Body Protection Compound): Enhances repair via VEGFR2/Akt-eNOS activation, increasing nitric oxide production and angiogenesis while stimulating fibroblast activity. This 15-amino-acid gastric peptide accelerates tendon, muscle, and gut repair.

KPV (Lys-Pro-Val): Provides anti-inflammatory effects by reducing NF-κB activation and attenuating pro-inflammatory cytokine signaling in epithelial and immune cells. This α-MSH-derived tripeptide complements the regenerative actions of TB-500 and BPC-157.

The combination thus addresses wound repair, angiogenesis, and inflammation simultaneously through distinct but synergistic pathways.

References and original studies

  • Annals of the New York Academy of Sciences (2012)

    The regenerative peptide thymosin β4 accelerates dermal healing in preclinical models and patients

    View original study

  • HSS Journal / PMC (2025)

    Emerging Use of BPC-157 in Orthopaedic Sports Medicine: A Systematic Review

    View original study

  • Molecular Therapy Methods & Clinical Development / PMC (2017)

    Orally Targeted Delivery of Tripeptide KPV via HA-Functionalized Nanoparticles Alleviates Ulcerative Colitis

    View original study

  • Frontiers in Endocrinology (2021)

    Progress on the Function and Application of Thymosin β4

    View source

  • Current Reviews in Musculoskeletal Medicine (2025)

    Regeneration or Risk? A Narrative Review of BPC-157 for Musculoskeletal Healing

    View original study

  • Journal of Orthopaedic Research (2003)

    Gastric pentadecapeptide BPC 157 accelerates healing of transected rat Achilles tendon and stimulates tendocyte growth

    View original study

  • CDC (Subcutaneous Injection PDF)

    Technique diagram and site guidance for subcutaneous injections

    View source

  • NCBI Bookshelf

    Best practices for injection: asepsis, preparation, and administration

    View original study

  • Subcutaneous Drug Injection Review / PMC

    Pharmacologic considerations of the subcutaneous route

    View original study

About the author

Vincent Ceniceros

Vincent Ceniceros created Peptide Companion and publishes its Dosage Library. Each guide summarizes a reference protocol and links to the studies and sources it draws on.

Published · Last updated

Related guides

Work out the mixing math for any vial with the peptide reconstitution calculator, or browse the full Dosage Library.

Educational content adapted from peptidedosages.com. Not medical advice.