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Peptide Companion
single
Healing & Recovery
Vial: 5 mg

TB-500 (5 mg Vial)

TB‑500 is a synthetic peptide fragment corresponding to the active region of thymosin beta‑4 (Tβ4), a naturally occurring 43‑amino‑acid protein involved in tissue repair and regeneration. This educational protocol presents a once‑daily subcutaneous approach using a practical dilution for accurate insulin‑syringe measurements in research settings.

Dose
2–5 mg per injection
Frequency
Twice weekly
Timing
Any time of day
Duration
4–8 weeks

Systemic tissue repair. Promotes cell migration and differentiation. Loading phase: 5mg 2x/week for 4 weeks.

Dosing Schedule
PhaseDaily Dose (mcg)Units (per injection) (mL)
Weeks 1–2500 mcg30 units (0.30 mL)
Weeks 3–4600 mcg36 units (0.36 mL)
Weeks 5–8750 mcg45 units (0.45 mL)
Weeks 9–121000 mcg60 units (0.60 mL)
Supplies Needed
  • •Peptide Vials (TB‑500, 5 mg each):
  • •Insulin Syringes (U‑100):
  • •Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.
  • •Alcohol Swabs: One for the vial stopper + one for the injection site each day.
References
  • FASEB Journal

    Biological activities of thymosin β4 defined by active peptide sequences (TB‑500 fragment identification)

    View source
  • Journal of Chromatography A (PubMed)

    Doping control analysis of TB‑500 as synthetic thymosin β4 fragment in biological samples

    View source
  • WADA Scientific Research

    Investigation of TB‑500 metabolism, synthesis of metabolites, and detection limits

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  • Racing Medication & Testing Consortium

    Thymosin β4 regulatory bulletin (TB‑500 use in equine sports medicine)

    View source
  • Journal of Investigative Dermatology (PubMed)

    Thymosin beta4 accelerates wound healing (preclinical wound healing model)

    View source
  • FASEB Journal (PubMed)

    Active site mapping of thymosin β4 fragments for angiogenesis and cell migration

    View source
  • Verified Peptides Storage Guide

    Lyophilized peptide storage best practices (temperature, humidity, light protection)

    View source
  • Empower Pharmacy

    Bacteriostatic water injection guidelines (0.9% benzyl alcohol, multi‑dose vial stability)

    View source
  • NCBI Bookshelf

    Best practices for subcutaneous injection (aseptic technique, site rotation)

    View source
  • WADA Prohibited List

    TB‑500 classification as prohibited substance in competitive sports

    View source
  • Journal of Investigative Dermatology

    Thymosin β4 wound healing mechanisms (collagen deposition, angiogenesis, granulation tissue)

    View source
  • Journal of Chromatography B (PubMed)

    Quantification of TB‑500 metabolites and wound healing activity screening (prodrug hypothesis)

    View source
  • CenterWatch Clinical Trials

    Clinical trial registry for thymosin β4 in acute myocardial infarction (no TB‑500 specific trials)

    View source
  • Nursing Journal (LWW)

    How to administer subcutaneous injections (clinical technique guidelines)

    View source
  • University of Michigan Health

    Patient education guide for subcutaneous injection technique

    View source

Educational content adapted from peptidedosages.com. Not medical advice.