
TB-500 (5 mg Vial)
TB‑500 is a synthetic peptide fragment corresponding to the active region of thymosin beta‑4 (Tβ4), a naturally occurring 43‑amino‑acid protein involved in tissue repair and regeneration. This educational protocol presents a once‑daily subcutaneous approach using a practical dilution for accurate insulin‑syringe measurements in research settings.
- Dose
- 2–5 mg per injection
- Frequency
- Twice weekly
- Timing
- Any time of day
- Duration
- 4–8 weeks
Systemic tissue repair. Promotes cell migration and differentiation. Loading phase: 5mg 2x/week for 4 weeks.
| Phase | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1–2 | 500 mcg | 30 units (0.30 mL) |
| Weeks 3–4 | 600 mcg | 36 units (0.36 mL) |
| Weeks 5–8 | 750 mcg | 45 units (0.45 mL) |
| Weeks 9–12 | 1000 mcg | 60 units (0.60 mL) |
- •Peptide Vials (TB‑500, 5 mg each):
- •Insulin Syringes (U‑100):
- •Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.
- •Alcohol Swabs: One for the vial stopper + one for the injection site each day.
FASEB Journal
Biological activities of thymosin β4 defined by active peptide sequences (TB‑500 fragment identification)
View sourceJournal of Chromatography A (PubMed)
Doping control analysis of TB‑500 as synthetic thymosin β4 fragment in biological samples
View sourceWADA Scientific Research
Investigation of TB‑500 metabolism, synthesis of metabolites, and detection limits
View sourceRacing Medication & Testing Consortium
Thymosin β4 regulatory bulletin (TB‑500 use in equine sports medicine)
View sourceJournal of Investigative Dermatology (PubMed)
Thymosin beta4 accelerates wound healing (preclinical wound healing model)
View sourceFASEB Journal (PubMed)
Active site mapping of thymosin β4 fragments for angiogenesis and cell migration
View sourceVerified Peptides Storage Guide
Lyophilized peptide storage best practices (temperature, humidity, light protection)
View sourceEmpower Pharmacy
Bacteriostatic water injection guidelines (0.9% benzyl alcohol, multi‑dose vial stability)
View sourceNCBI Bookshelf
Best practices for subcutaneous injection (aseptic technique, site rotation)
View sourceWADA Prohibited List
TB‑500 classification as prohibited substance in competitive sports
View sourceJournal of Investigative Dermatology
Thymosin β4 wound healing mechanisms (collagen deposition, angiogenesis, granulation tissue)
View sourceJournal of Chromatography B (PubMed)
Quantification of TB‑500 metabolites and wound healing activity screening (prodrug hypothesis)
View sourceCenterWatch Clinical Trials
Clinical trial registry for thymosin β4 in acute myocardial infarction (no TB‑500 specific trials)
View sourceNursing Journal (LWW)
How to administer subcutaneous injections (clinical technique guidelines)
View sourceUniversity of Michigan Health
Patient education guide for subcutaneous injection technique
View source
Educational content adapted from peptidedosages.com. Not medical advice.