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Healing & Recovery
Vial: 50 mg

GHK-Cu (50 mg Vial)

GHK-Cu (glycyl-L-histidyl-L-lysine:copper complex) is a naturally occurring copper peptide with documented roles in wound healing, tissue remodeling, and skin regeneration. Research demonstrates activity in gene regulation related to collagen synthesis, antioxidant defense, and anti-inflammatory pathways. This educational protocol presents practical subcutaneous administration approaches based on clinical practice patterns.

Dose
1–3 mg per injection
Frequency
Once daily or every other day
Timing
Evening preferred
Duration
8–12 weeks

Copper peptide. Promotes collagen synthesis and wound healing. Anti-inflammatory effects.

Dosing Schedule
Week/PhaseDose per InjectionUnits (per injection) (mL)
Weeks 1–41.0 mg (1000 mcg)6 units (0.06 mL)
Weeks 5–81.5 mg (1500 mcg)9 units (0.09 mL)
Weeks 9–12+2.0 mg (2000 mcg)12 units (0.12 mL)
Supplies Needed
  • •Peptide Vials (GHK-Cu, 50 mg each):
  • •Insulin Syringes (U‑100, 29–31 gauge):
  • •Sterile or Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.
  • •Alcohol Swabs: One for the vial stopper + one for the injection site each administration.
References

  • Journal of Dermatological Science

    GHK-Cu stimulates collagen synthesis in dermal fibroblasts and wound healing processes

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  • Wound Repair and Regeneration (Wiley)

    Copper peptides in wound healing: tissue remodeling and collagen gene expression

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  • International Journal of Molecular Sciences (PMC)

    Regenerative and protective actions of GHK-Cu: gene regulation analysis

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  • Biomaterials (Elsevier)

    GHK modulation of metalloproteinases and tissue inhibitors in wound healing

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  • Dr Oracle AI

    Clinical dosing patterns for GHK-Cu (1–2 mg subcutaneous protocols)

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  • Salhab Pharmacy (Compounding Guidelines)

    GHK-Cu injection protocols: 5 days/week and 3×/week dosing patterns

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  • GenScript

    Peptide storage and handling guidelines: lyophilized storage at −20 °C (−4 °F)

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  • Peptide Sciences

    Peptide storage best practices: temperature, humidity, and light protection

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  • Happy Hormones MD (PDF)

    GHK-Cu patient information: reconstituted storage at 2–8 °C (35.6–46.4 °F), 30-day use window

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  • Tocris Bioscience

    Stability and storage: aliquoting peptide solutions to minimize freeze-thaw damage

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  • GenScript

    Avoiding repeated freeze-thaw cycles to prevent peptide degradation

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  • Roswell Park Comprehensive Cancer Center

    Injection site rotation to prevent lipodystrophy and local irritation

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  • Roswell Park Comprehensive Cancer Center

    Subcutaneous injection site selection: abdomen, thighs, upper arms

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  • Roswell Park Comprehensive Cancer Center

    Proper skin preparation: alcohol swab cleaning and air-drying before injection

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  • International Journal of Molecular Sciences (PMC)

    Preclinical studies: GHK-Cu activity at low doses (~0.5 mcg/kg in rats)

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  • Brain Sciences (MDPI)

    GHK effect on gene expression relevant to nervous system function and cognitive decline

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  • CDC

    Vaccine administration: subcutaneous injection technique (angle, no aspiration)

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  • CDC (Subcutaneous Injection PDF)

    Technique diagram and site guidance for subcutaneous injections

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  • NCBI Bookshelf

    Best practices for injection: asepsis, preparation, administration, and sharps disposal

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Educational content adapted from peptidedosages.com. Not medical advice.