Cortagen (20 mg Vial)

Cortagen dosage protocols are designed around this synthetic tetrapeptide (Ala-Glu-Asp-Pro) that belongs to the Khavinson bioregulator class, studied for its neuroprotective and neuroregenerative properties. Research indicates Cortagen may support peripheral nerve regeneration, enhance cognitive function, reduce oxidative stress in neural tissues, and promote cellular repair mechanisms without significant adverse effects at therapeutic doses. This educational protocol presents a once-daily subcutaneous approach using a practical dilution for clear insulin-syringe measurements.

Detailed protocol below.

Dosing Schedule

Week	Daily Dose (mcg)	Units (per injection) (mL)
Weeks 1–2	1000 mcg	15 units (0.15 mL)
Weeks 3–4	2000 mcg	30 units (0.30 mL)

Supplies Needed

•Peptide Vials (Cortagen, 20 mg each):
•Insulin Syringes (U-100):
•Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution.
•Alcohol Swabs: One for the vial stopper + one for the injection site each day.

References

PubMed
Effect of tetrapeptide cortagen on regeneration of sciatic nerve (nerve growth rate and conduction velocity improvements)
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PubMed
Cortexin and cortagen as correcting agents in functional and metabolic disorders in the brain in chronic ischemia
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PubMed (Neuroendocrinology Letters)
Elucidation of the effect of brain cortex tetrapeptide Cortagen on gene expression in mouse heart by microarray
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PubMed (Biogerontology)
Peptide bioregulation of aging: results and prospects (lifespan extension and biomarker modulation)
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Bentham Open (Open Neuropsychopharmacology Journal)
Modulatory effects of Cortexin and Cortagen on locomotor activity and anxiety-related behavior in mice
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MDPI (Molecules)
Peptide Regulation of Gene Expression: A Systematic Review (epigenetic mechanisms)
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Springer (Bulletin of Experimental Biology and Medicine)
Mechanisms Underlying Geroprotective Effects of Peptides
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MedlinePlus
Subcutaneous (SQ) injections: technique, site rotation, and best practices
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CDC (Subcut Injection PDF)
Vaccine Administration: Subcutaneous (SUBCUT) Injection technique and guidance
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Bachem
Handling and Storage Guidelines for Peptides (stability and storage conditions)
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MDPI (International Journal of Molecular Sciences)
Short Peptides Protect Fibroblast-Derived Induced Neurons from Age-Related Changes
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Wikipedia
Cortagen (tetrapeptide overview and background)
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Educational content adapted from peptidedosages.com. Not medical advice.