Peptide Companion
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Peptide Companion
single
Cognitive
Vial: 10 mg

PE-22-28 (10 mg Vial)

PE-22-28 is a synthetic heptapeptide (sequence: GVSWGLR) derived from the sortilin propeptide, engineered as a potent and selective antagonist of TREK-1 potassium channels. Preclinical studies demonstrate rapid antidepressant-like effects, enhanced neurogenesis, and neuroprotection with a favorable safety profile showing no cardiac or metabolic side effects. This educational protocol presents a once-daily subcutaneous approach with gradual titration.

Detailed protocol below.

Dosing Schedule
WeekDaily Dose (µg)Units (per injection) (mL)
Weeks 1–250 µg1.5 units (0.015 mL)
Weeks 3–4100 µg3 units (0.03 mL)
Weeks 5–8100 µg3 units (0.03 mL)
Weeks 9–12 (Optional)150 µg4.5 units (0.045 mL)
Weeks 13–16 (Optional)200 µg6 units (0.06 mL)
Supplies Needed
  • •Peptide Vials (PE-22-28, 10 mg each):
  • •Insulin Syringes (U-100):
  • •Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.
  • •Alcohol Swabs: One for the vial stopper + one for the injection site each day.
References

  • PLoS Biology (2010)

    Mazella et al.: Spadin, a sortilin-derived peptide targeting TREK-1 channels; novel antidepressant mechanism

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  • Neuropharmacology (2012)

    Moha Ou Maati et al.: Spadin as antidepressant; absence of TREK-1-related side effects

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  • Frontiers in Pharmacology (2017)

    Djillani et al.: Shortened spadin analogs (PE-22-28); superior TREK-1 inhibition, in vivo stability, antidepressant activity

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  • Neuropharmacology (2019)

    Pietri et al.: Protective effects on stroke recovery and post-stroke depression induced by sortilin-derived peptides

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  • Pharmacological Research (2021)

    Daziano et al.: Sortilin-derived peptides promote pancreatic beta-cell survival through CREB signaling pathway

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  • International Journal of Molecular Sciences (2022)

    Chang et al.: Potential of heterogeneous compounds as antidepressants; narrative review including TREK-1 modulators

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  • CDC

    Vaccine administration: subcutaneous injection instruction guide (technique, angle, no aspiration)

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  • CDC

    4 Ways to Take Insulin; section on injection site rotation and subcutaneous technique

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Educational content adapted from peptidedosages.com. Not medical advice.