NAD+ (500 mg Vial)
NAD+ (nicotinamide adenine dinucleotide) is a critical coenzyme involved in cellular energy metabolism, DNA repair, and mitochondrial function. Clinical research has primarily employed intravenous infusions at high doses, though subcutaneous administration at lower doses is emerging as a practical maintenance route. This educational protocol presents a once‑daily subcutaneous approach with gradual titration for improved tolerability.
- Dose
- 50–250 mg per injection
- Frequency
- 2–3 times weekly
- Timing
- Morning preferred
- Duration
- Ongoing as tolerated
Cellular energy metabolism. May cause flushing. Start low and increase gradually.
| Week | Daily Dose (mg) | Units (per injection) (mL) |
|---|---|---|
| Week 1 | 50 mg | 30 units (0.30 mL) |
| Week 2 | 75 mg | 45 units (0.45 mL) |
| Weeks 3–8 | 100 mg | 60 units (0.60 mL) |
| Weeks 9–12 | 100 mg | 60 units (0.60 mL) |
| Weeks 13–16 | 100 mg | 60 units (0.60 mL) |
- •Peptide Vials (NAD+, 500 mg each):
- •Insulin Syringes (U‑100, 1 mL):
- •Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution.
- •Alcohol Swabs: One for the vial stopper + one for the injection site each day.
Pharmaceuticals (Basel)
Clinical evidence for targeting NAD+ therapeutically: metabolic pathways and therapeutic potential
View sourceCurrent Psychiatry Research and Reviews
NAD+ and enkephalinase infusions attenuate burden in substance use disorder (pilot of 50 cases)
View sourceFagron Academy (US)
NAD+ dosing review: routes, protocols, and case study insights from clinical practice
View sourceJinfiniti (NAD+ Dosage Chart)
NAD+ injection dosage guidance: chart, timing, and safety considerations for titration
View sourceOlympia Pharmacy
NAD+ injection (Nicotinamide Adenine Dinucleotide): compounding pharmacy information and dosing guidance
View sourceSigma-Aldrich Product Information
β‑Nicotinamide adenine dinucleotide (NAD+) product specification and stability data
View sourceEmpower Pharmacy
NAD+ injection (lyophilized): compounding specifications and reconstitution guidance
View sourcePMC (Pharmacology & Potential Implications of NAD+)
Comprehensive review of NAD+ metabolism, aging, and therapeutic applications
View sourceAgelessRx
NAD+ injection clinical overview and administration protocols
View sourceFrontiers in Aging Neuroscience
A pilot study investigating changes in human plasma and urine NAD+ metabolome during 6‑hour IV infusion (750 mg dose)
View sourcePure Bio Labs
NAD+ vial (500 mg): high‑purity peptide product specifications
View sourceFagron Academy (NAD+ Review)
Subcutaneous and intramuscular NAD+ administration: emerging practices for maintenance therapy
View sourceJinfiniti (Dosage Chart)
Human case reports: benefits at 100–300 mg/day ranges for cognitive and metabolic support
View sourceAmerican Journal of Physiology (PubMed)
Evaluation of safety and effectiveness of NAD+ in different clinical conditions: a systematic review
View sourceFagron Academy
Dosing considerations: regimens exceeding 200–300 mg/day reserved for supervised therapeutic use
View sourceCDC Pink Book (Chapter 6)
Vaccine administration: needle selection and injection technique for subcutaneous route
View sourceCDC Subcutaneous Injection Guide
Technique diagram and site guidance for subcutaneous injections (45–90° angle, no aspiration)
View sourceNCBI Bookshelf
Best practices for injections: asepsis, site preparation, administration, and rotation to prevent lipohypertrophy
View sourcePMC (Subcutaneous Drug Injection Review)
Literature review of factors influencing pain at injection site and volume considerations for subcutaneous route
View source
Educational content adapted from peptidedosages.com. Not medical advice.