Peptide Companion
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Recovery & Repair
Vial: 5 mg

How is TB-500 (5 mg) + BPC-157 (5 mg) Stack dosed and mixed?

TB-500 (5 mg) + BPC-157 (5 mg) Stack is a peptide stack research reference in the Recovery & Repair category for a 5 mg vial. The reference mixing guide adds 2 mL of bacteriostatic water, giving a concentration of 2.5 mg/mL. The published research schedule lists 0.25–0.5 mg per injection, once or twice daily for 4–8 weeks; it is reference information, not a recommendation.

By Vincent Ceniceros · Published · Last updated

TB-500 (Thymosin β4) is a 43-amino-acid peptide that promotes tissue repair and regeneration through enhanced angiogenesis and cellular migration. BPC-157 (Body Protection Compound-157) is a 15-amino-acid gastric peptide studied for accelerating musculoskeletal repair by upregulating growth factor signaling. Stacking these peptides is a common research approach for synergistic tissue repair support.

Dose
0.25–0.5 mg per injection
Frequency
Once or twice daily
Timing
Morning and/or evening
Duration
4–8 weeks

Systemic repair effects. Can be injected near injury site or subcutaneously. Promotes angiogenesis.

Dosing Schedule
WeekDose (mcg)Units (per injection) (mL)Frequency
Weeks 1–41,250 mcg (1.25 mg)50 units (0.50 mL)Twice weekly
Weeks 5–121,250 mcg (1.25 mg)50 units (0.50 mL)Once weekly
Mixing & Quick Reference
Bacteriostatic water
2 mL
Concentration
2.5 mg/mL
  • •TB-500 Reconstitution: Add 2.0 mL bacteriostatic water → 2.5 mg/mL concentration.
  • •BPC-157 Reconstitution: Add 2.0 mL bacteriostatic water → 2.5 mg/mL concentration.
  • •TB-500 Dosing: 1,250 mcg (50 units) twice weekly (Weeks 1–4), then once weekly (Weeks 5–12).
  • •BPC-157 Dosing: 250–500 mcg (10–20 units) daily, titrated over 12 weeks.
  • •Easy measuring: At 2.5 mg/mL, 1 unit = 0.01 mL = 25 mcg on a U-100 insulin syringe.
  • •Storage: Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F); after reconstitution, refrigerate and use within ~30 days.
Supplies Needed
  • •TB-500 Vials (5 mg each):
  • •BPC-157 Vials (5 mg each):
  • •Insulin Syringes (U-100):
  • •Bacteriostatic Water (10 mL bottles): Use 2.0 mL per vial for reconstitution.
  • •Alcohol Swabs: One for the vial stopper + one for the injection site each administration.
Benefits
  • •TB-500: Preclinical studies show accelerated wound closure, reduced fibrosis, and improved function after ischemic or traumatic injuries.
  • •BPC-157: Preclinical models demonstrate accelerated recovery of tendon, ligament, muscle, and bone injuries with no observed toxicity. One clinical series found pain relief in 7 of 12 patients after a single intraarticular knee injection.
  • •Stack Rationale: The combination may offer complementary mechanisms—systemic repair signaling (TB-500) plus localized tissue repair (BPC-157).
  • •Side Effects: Generally well tolerated in research settings; occasional mild injection-site reactions (redness, itching) may occur with subcutaneous administration.
How It Works

TB-500 mimics endogenous thymosin β4, modulating inflammation and activating repair pathways including PI3K/Akt and Notch signaling to enhance angiogenesis and cell migration. It promotes actin sequestration and is studied for accelerating wound repair, reducing fibrosis, and improving function after ischemic or traumatic injuries.

BPC-157 upregulates angiogenic pathways (including VEGF) and counteracts inflammatory cytokines. Preclinical research demonstrates improved repair of muscle, tendon, ligament, and bone with no observed toxicity. The combination targets complementary mechanisms: TB-500 for systemic tissue repair signaling and BPC-157 for localized repair support.

References and original studies

  • Frontiers in Endocrinology (2021)

    Progress on the Function and Application of Thymosin β4

    View original study

  • Annals of the New York Academy of Sciences

    Thymosin β4: roles in development, repair, and engineering of the cardiovascular system

    View original study

  • Frontiers in Pharmacology (2022)

    Pharmacokinetics, distribution, metabolism, and excretion of BPC-157

    View original study

  • HSS Journal (2025)

    Emerging Use of BPC-157 in Orthopaedic Sports Medicine: A Systematic Review

    View original study

  • Current Pharmaceutical Design (2018)

    Stable Gastric Pentadecapeptide BPC 157: Novel Therapy in Gastrointestinal Tract

    View original study

  • Journal of Orthopaedic Surgery and Research (2019)

    BPC 157 and its effects on tendon healing

    View original study

  • CDC (2024)

    Vaccine Administration: Subcutaneous (SUBCUT) Injection

    View source

  • NCBI Bookshelf (2023)

    Administration of Parenteral Medications – Nursing Skills

    View original study

About the author

Vincent Ceniceros

Vincent Ceniceros created Peptide Companion and publishes its Dosage Library. Each guide summarizes a reference protocol and links to the studies and sources it draws on.

Published · Last updated

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Work out the mixing math for any vial with the peptide reconstitution calculator, or browse the full Dosage Library.

Educational content adapted from peptidedosages.com. Not medical advice.